LOCUS       40924_41395        3724 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSU30 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Goto K, Nagano Y.
  TITLE     Ultra-low background DNA cloning system
  JOURNAL   PLoS ONE 8 (2), E56530 (2013)
  PUBMED    23409191
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Nagano Y, Goto K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2012) Contact:Yukio Nagano Saga University, 
            Analytical Research Center for Experimental Sciences; 1 Honjo-machi,
            Saga 840-8502, Japan
REFERENCE   3  (bases 1 to 3724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "2"; pages: "E56530"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-OCT-2012) Contact:Yukio Nagano Saga University, Analytical 
            Research Center for Experimental Sciences; 1 Honjo-machi, Saga 
            840-8502, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(1030..1533)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        1542..1823
                     /label=TRP1 promoter
     CDS             1824..2495
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(2651..2667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2675..2691)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2699..2729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2744..2765)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3053..3641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aacaattcgt tcaagccgag atcggcttcc
     1021 cggccgacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
     1081 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
     1141 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
     1201 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
     1261 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
     1321 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
     1381 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
     1441 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
     1501 atttaaatta taattatttt tatagcacgt gatgaaagga ccacgacatt actatatata
     1561 taatatagga agcatttaat agaacagcat cgtaatatat gtgtactttg cagttatgac
     1621 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
     1681 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
     1741 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
     1801 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
     1861 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
     1921 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
     1981 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
     2041 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
     2101 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
     2161 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
     2221 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
     2281 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
     2341 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
     2401 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
     2461 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
     2521 ttaagtattg tttgtgcact tgccctgtca gaccaagttt actcatatat actttagatt
     2581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgatgaattc
     2641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaggg
     3721 atcc
//
